کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2394295 | 1551527 | 2014 | 10 صفحه PDF | دانلود رایگان |
Amniotic membrane as a biocompatible and permi-selective immune barrier was harvested from full-term pregnant bitches and cryopreserved by using Dulbecco’s modified Eagle’s medium (DMEM) or Minimal essential medium (MEM) 50% medium in glycerol 50% at temperature below freezing −80 °C. A defined 3 cm × 3 cm patch in the craniodorsal surface of the bladder was excised and then autografted in 15 apparent healthy mongrel dogs. In the place of excised patches of 27 apparent healthy mongrel dogs, double layers of 4 cm × 5 cm cryopreserved amniotic membranes were implanted. The results of plain and contrast radiography, ultrasonography, blood and urine analysis and histopathology confirmed that urinary bladder surgical defect that replaced by amniotic membrane implant, proved successful revival by normal structural, functional and contractile layers without any growth abnormalities throughout three months period. The successful implantation model of amniotic membrane will encourage its use on human beings for renovation in the surgical management of bladder cancers and restoring damaged or diseased urinary bladders.
Journal: International Journal of Veterinary Science and Medicine - Volume 2, Issue 1, June 2014, Pages 57–66