کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2428807 1553572 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
C5a of Cynoglossus semilaevis has anaphylatoxin-like properties and promotes antibacterial and antiviral defense
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
C5a of Cynoglossus semilaevis has anaphylatoxin-like properties and promotes antibacterial and antiviral defense
چکیده انگلیسی


• Bacterial and viral infection enhanced CsC5 expression in immune organs.
• Recombinant CsC5a (rCsC5a) induced chemotaxis of peripheral blood leukocytes (PBL).
• rCsC5a was able to bind and activate PBL.
• rCsC5a enhanced the phagocytosis of PBL.
• rCsC5a enhanced resistance against bacterial and viral infection.

Activation of the complement system leads to the cleavage of component factor C5 into C5a and C5b. C5a can induce chemotaxis and inflammatory responses in mammals. The function of C5a in fish is poorly understood. In this study, we report the identification and analysis of a C5 homologue, CsC5, from tongue sole (Cynoglossus semilaevis). CsC5 is composed of 1683 amino acid residues that include an anaphylatoxin homologous domain. Expression of CsC5 could be detected in a variety of tissues and was up-regulated by bacterial or viral pathogen infection. Purified recombinant CsC5a (rCsC5a) could bind to peripheral blood leukocytes (PBL) and stimulate PBL chemotaxis, proliferation, respiratory burst, acid phosphatase activity, and phagocytosis. Tongue sole administered rCsC5a exhibited enhanced resistance against bacterial and viral infections. These results indicate that CsC5a is an anaphylatoxin with a role in innate immune defense against bacterial and viral infections.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 60, July 2016, Pages 139–148
نویسندگان
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