کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429047 1106472 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular cloning, expression, purification and characterization of vitellogenin in scallop Patinopecten yessoensis with special emphasis on its antibacterial activity
ترجمه فارسی عنوان
شبیه سازی مولکولی، بیان، تصفیه و مشخص کردن ویلیوگنین در پاتینوپکتن یسوئنس، با تأکید ویژه بر فعالیت آنتی باکتریال
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


• Full length Vg gene in P. yessoensis is cloned and native Vg protein is purified.
• PyVg gene is predominantly distributed in the ovary and hepatopancreas.
• PyVg is involved in the acute phase response.
• PyVg is a pattern recognition molecule.

Vitellogenin (Vg), the major precursor of the egg-yolk proteins, has been found to play an immune role in fish and protochordate amphioxus, however, no study on the immune function of Vg in invertebrates has ever been studied before. In this study, the complete cDNA of Vg was identified from the scallop Patinopecten yessoensis (termed PyVg). The cDNA contained an open reading frame (ORF) of 6888 bp, encoding a polypeptide of 2295 amino acid protein, which had an N-terminal signal peptide followed by the mature Vg. The mature Vg had the domains Vitellogenin_N, domain of unknown function 1943 (DUF1943) and von Willebrand factor type D domain (VWD) as well as the consensus cleavage site (R-X-R/K-R) and conserved motif (KTIGNAG). Tissue distribution assay revealed that PyVg transcripts were predominantly present in the ovary and hepatopancreas, and its expression profile in ovary well reflected the annual cycle of vitellogenesis. Interestingly, bacterial challenge caused a significant change in PyVg expression, hinting an involvement of PyVg in the acute phase response in P. yessoensis. Consistently, recombinant DUF1943 and VWD domains both could interact with LTA and LPS on bacterial wall, and purified native PyVg displayed a broad-spectrum antibacterial activity against both Gram-negative (Escherichia coli and Vibrio anguillarum) and Gram-positive bacteria (Staphylococcus aureus). Overall, these data indicate that Vg is a pattern recognition molecule with bacterial growth-inhibiting activity in the scallop.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 49, Issue 2, April 2015, Pages 249–258
نویسندگان
, , , , ,