کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2429137 | 1106479 | 2014 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of a C-type lectin with antiviral and antibacterial activity from pacific white shrimp Litopenaeus vannamei Identification of a C-type lectin with antiviral and antibacterial activity from pacific white shrimp Litopenaeus vannamei](/preview/png/2429137.png)
• A C-type lectin LvCTL3 from Litopenaeus vannamei.
• NF-κB signaling pathway regulates expression of LvCTL3.
• LvCTL3 shows different agglutination activities for different bacteria.
• LvCTL3 inhibits bacterial or viral infection in vivo.
C-type lectins (CTLs) play crucial roles in innate immune responses in invertebrates by recognizing and eliminating microinvaders. In this study, a CTL from pacific white shrimp Litopenaeus vannamei (LvCTL3) was identified. LvCTL3 contains a single C-type lectin-like domain (CTLD), which shows similarities to those of other shrimp CTLs and has a mutated ‘EPD’ motif in Ca2+-binding site 2. LvCTL3 mRNA can be detected in all tested tissues and expression of LvCTL3 in gills was up-regulated after Lipopolysaccharides, poly (I:C), Vibrio parahaemolyticus and white spot syndrome virus (WSSV) challenges, suggesting activation responses of LvCTL3 to bacterial, virus and immune stimulant challenges. The 5′flanking regulatory region of LvCTL3 was cloned and we identified a NF-κB binding motif in the LvCTL3 promoter region. Dual-luciferase reporter assays indicated that over-expression of L. vannamei dorsal can dramatically up regulate the promoter activity of LvCTL3, suggesting that LvCTL3 expression could be regulated through NF-κB signaling pathway. As far as we know, this is the first report on signaling pathway involve in shrimp CTLs expression. The recombinant LvCTL3 protein was expressed in Escherichia coli and purified by Ni-affinity chromatography. The purified LvCTL3 can agglutinate Gram-negative microbe Vibrio alginolyticus and V. parahaemolyticus and Gram-positive bacteria Bacillus subtilis in the presence of calcium ions, but cannot agglutinate Gram-positive bacteria Streptococcus agalactiae. The agglutination activity of LvCTL3 was abolished when Ca2+ was chelated with EDTA, suggesting the function of LvCTL3 is Ca2+-dependent. In vivo challenge experiments showed that the recombinant LvCTL3 protein can significantly reduce the mortalities of V. parahemolyticus and WSSV infection, indicating LvCTL3 might play significant roles in shrimp innate immunity defense against bacterial and viral infection.
Journal: Developmental & Comparative Immunology - Volume 46, Issue 2, October 2014, Pages 231–240