Antiviral activity of phosvitin from zebrafish Danio rerio

Lymphocystis disease virus (LCDV), a virus of Iridoviridae, can infect numerous teleost species, causing serious losses of aquaculture industry, and thus effective ways of prophylaxis and treatment are demanded. Previous studies have shown that phosvitin (Pv) is an antimicrobial agent in zebrafish, and vitellogenin, the precursor of yolk proteins including Pv, is able to neutralize virus, we thus hypothesize that Pv may have an antiviral activity. Here we clearly demonstrated that recombinant Pv (rPv) purified was capable of inhibiting the cytopathic effect in LCDV-infected cells and reducing the virus quantities in the infected cells as well as in the infected zebrafish. These data indicate that Pv possesses an antiviral activity and participates in immune defense of host against the infection by viruses like LCDV.

► Pv was capable of inhibiting the cytopathic effect in LCDV-infected cells.
► Pv markedly reduced the relative quantities of LCDV in the FG-9307 cells infected by LCDV.
► Pv caused a significant decrease in the relative quantity and proliferation of LCDV in zebrafish.