کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429406 1106494 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of new allotypic variants of bovine λ-light chain constant regions in different cattle breeds
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Detection of new allotypic variants of bovine λ-light chain constant regions in different cattle breeds
چکیده انگلیسی

In the cattle breeds German Black Pied (GBP), German Simmental (GS), Holstein Friesian (HF), Aubrac (A) three transcribed allotypic variants in isotype IGLC2 and five allotypic variants in isotype IGLC3 were identified. Substitutions within the putative interface to CH1 at position 11 and 79 were noted. In IGLC2b, K79E led to a charge conversion. In IGLC3b and IGLC3c, the E79N replacement removed the charge while the T11K substitution resulted in a positively charged amino acid residue. In addition, D15 and T16 were found in IGLC2c, IGLC3b, and IGLC3c. Substitutions located on the outer site of the molecule were observed in IGLC2b (V40, H45.5), IGLC2c (A1, V40, D77), IGLC3b (A1, D77, D109, P127), IGLC3c (A1, G45.5, D77, D109, P127), IGLC3d (D109), and IGLC3e (A1). Amino acid residues P83 (IGLC2c, IGLC3b, IGLC3c), N93 (IGLC2b), D93 (IGLC3b), and G93 (IGLC3c) were positioned in cavities but seemed to be accessible for solvents.


► Allotypic variants of transcribed λ-light chain isotypes were detected in cattle.
► Three allotypic variants were found for IGLC2.
► Five allotypic variants were found for IGLC3.
► Analyses in different cattle breeds of diverse economic impacts revealed different distributions of allotypic variants.
► Fundamental study for the investigation of association between allotypic markers and susceptibility to infectious diseases as described already for humans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 36, Issue 1, January 2012, Pages 130–139
نویسندگان
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