کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429497 1106500 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Porcine CD27: Identification, expression and functional aspects in lymphocyte subsets in swine
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Porcine CD27: Identification, expression and functional aspects in lymphocyte subsets in swine
چکیده انگلیسی

Up to now for Swine Workshop Cluster 2 (SWC2) the orthologous human CD molecule was unknown. By use of the SWC2-specific mAb b30c7 and a retroviral cDNA expression library derived from stimulated porcine peripheral blood mononuclear cells we could identify SWC2 as porcine CD27. Phenotypic analyses of lymphocytes isolated from blood and lymphatic organs revealed that mature T cells in thymus and T cells in the periphery with a naïve phenotype were CD27+. However, within CD8α+ T helper and CD8α+ γδ T cells also CD27− cells were present, indicating a down-regulation after antigen contact in vivo. B cells lacked CD27 expression, whereas NK cells expressed intermediate levels. Furthermore, plate-bound mAb b30c7 showed a costimulatory capacity on CD3-activated T cells for proliferation, IFN-γ and TNF-α production. Hence, our data indicate an important role of porcine CD27 for T-cell differentiation and activation as described for humans and mice.


► First identification of porcine CD27 by a mAb and a cDNA expression library.
► Porcine B cells were found to be CD27 negative.
► Mature thymocytes, T cells with a naïve phenotype and most NK cells expressed CD27.
► Subpopulations of T cells with a memory phenotype were CD27 negative.
► The CD27-specific mAb costimulated T cells for proliferation and cytokine production.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 38, Issue 2, October 2012, Pages 321–331
نویسندگان
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