کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2429606 | 1106508 | 2012 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response](/preview/png/2429606.png)
Lineage negative dendritic cells, or natural interferon-producing cells (NIPC), also referred to as plasmacytoid dendritic cells (pDC) constitute a small population of leukocytes secreting high levels of type I interferon (IFNα/β) in response to certain danger signals. Here, we provide initial data towards the identification of so far uncharacterised circulating bovine pDC like cells. A lineage negative cell population (LIN− cells) was isolated from PBMC which showed characteristics similar to that of pDC in other species. Isolated LIN− cells presented lymphoid morphology with a semi-crescent nucleus, extensive ER and Golgi network; indicative of pDC. In addition phenotypic analysis of LIN− cells described them as distinct from other bovine DC subsets; expressing both lymphoid and myeloid surface markers. LIN− cells did not express lineage specific markers, but were MHC class II+, CD45RO+, CD80/86+, CD6+, WC1+, CD26+ and expressed the myeloid markers CD205, CD172a and CD11a. In keeping with pDC, LIN− cells express TLR7 mRNA transcripts; however, in a resting state do not express TLR8 or TLR9. Functionally, LIN− cells, but not PBMC, monocytes and monocyte derived DC produce large amounts of IFNα/β in response to different CpG oligonucleotides. Taken together, we present data suggesting that an enriched circulating population of bovine LIN− cells are uniquely capable of producing IFNα/β in response to CpG oligonucleotides and thus this population likely contain the functional equivalent of bovine pDC.
► We have identified bovine LIN− cells, which may represent the bovine equivalent of pDC.
► We characterise these cells by flow cytometry, SEM and TEM.
► We show that the isolated LIN− cells respond to different CpG ODN with type-I IFN production.
Journal: Developmental & Comparative Immunology - Volume 36, Issue 2, February 2012, Pages 332–341