کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2429608 | 1106508 | 2012 | 10 صفحه PDF | دانلود رایگان |
Delayed-type hypersensitivity (DTH) response mediated by antigen-specific Th1 cells is used as a test to detect exposure to tuberculosis in humans. Japanese flounder (Paralichthys olivaceus) microtubule aggregate protein homologue (PoMTAP) was identified as a gene strongly induced during fish DTH response. In this study, PoMTAP gene was cloned and its expression profile was analyzed. The PoMTAP gene has a transcriptional regulatory region that includes two interferon-stimulated response elements and two IFN-γ activated sites. Expressions of PoMTAP and IFN-γ genes were up-regulated at the same time points during the DTH response, Edwardsiella tarda infection and VHSV infection. Furthermore, PoMTAP gene expressing cells also expressed CD3ε, confirming that PoMTAP is expressed by T lymphocytes. These results suggest that PoMTAP is a novel immune-related gene expressed by T lymphocytes that is preferentially induced by IFN-γ and has a role in Th1-mediated immune responses in Japanese flounder.
► Japanese flounder microtubule aggregate protein homologue (PoMTAP) gene were cloned.
► The promoter region of PoMTAP contains ISREs, GASs and C/EBP elements.
► The expression patterns of PoMTAP gene were similar to those of IFN-γ.
► PoMTAP gene expressing cells also expressed CD3ε.
► PoMTAP gene might be an IFN-γ-induced gene expressed by T lymphocytes and has a role in Th1-mediated immune responses.
Journal: Developmental & Comparative Immunology - Volume 36, Issue 2, February 2012, Pages 349–358