β-1,3-Glucan recognition protein (βGRP) is essential for resistance against fungal pathogen and opportunistic pathogenic gut bacteria in Locusta migratoria manilensis

Pattern recognition proteins, which form part of the innate immune system, initiate host defense reactions in response to pathogen surface molecules. The pattern recognition protein β-1,3-glucan recognition protein (βGRP) binds to β-1,3-glucan on fungal surfaces to mediate melanization via the prophenoloxidase (PPO)-activating cascade. In this study, cDNA encoding a 53-kDa βGRP (LmβGRP) was cloned from Locusta migratoria manilensis. LmβGRP mRNA shown to be constitutively expressed specifically in hemocytes and was highly upregulated following fungal infection. LmβGRP-silenced (LmβGRP-RNAi) mutant locusts exhibited significantly reduced survival rate following fungal infection (Metarhizium acridum) compared with the wild-type. Furthermore, LmβGRP-RNAi mutants exhibited abnormally loose stools indicative of a gut defect. 16S rRNA gene analysis detected the opportunistic pathogenic bacterium, Vibrio vulnificus in LmβGRP mutant but not wild-type locusts, suggesting changes in the composition of gut bacterial communities. These results indicate that LmβGRP is essential to gut immunity in L. migratoria manilensis.

► A 53-kDa βGRP (LmβGRP) was cloned from Locusta migratoria manilensis.
► LmβGRP mRNA is expressed in hemocytes and upregulated by fungal infection.
► LmβGRP-RNAi mutant locusts exhibit increased mortality following fungal infection.
► LmβGRP-RNAi mutant locusts exhibit altered gut bacterial communities.
► LmβGRP is essential to gut immunity in L. migratoria manilensis.