Identification and characterization of a Tegillarca granosa ferritin regulated by iron ion exposure and thermal stress

Ferritin, a conserved iron storage protein of most living organisms, plays a crucial role in iron metabolism. In this study, the ferritin gene from Tegillarca granosa (denoted as TgFER) was identified by expressed sequence tag (EST) and PCR approaches. The full-length cDNA of TgFER was of 895 bp, consisting of a 5′-UTR of 163 bp with a putative iron regulatory element (IRE), a 3′-UTR of 213 bp, and a complete open reading frame of 519 bp encoding a polypeptide with 172 amino acid residues. The predicted molecular mass of deduced amino acid of TgFER was 20.00 kDa and the theoretical pI was 4.89. The deduced amino acid of TgFER shared high identities to ferritin from abalone, oyster, clam and human. The tissue distribution of TgFER in the tissues of mantle, foot, gill, haemocytes and hepatopancreas was examined by quantitative real-time PCR (q-PCR) and mRNA transcripts of TgFER were found to be dominately expressed in haemocytes, hepatopancreas and gill and weakly in foot and mantle. The temporal expression of TgFER in haemocytes or hepatopancreases after challenged by metals ion (FeCl2 and FeCl3) exposure and thermal stress were also analyzed with q-PCR. The diverse expression patterns of TgFER were detected depending upon the types of stimulators and tissues. The ployconal antibodies generated from the recombinant product of TgFER could be specifically identified not only the recombinant product, but also the native protein from haemocytes. All these results strongly suggested that TgFER was involved in the iron metabolism and thermal stress regulation in T. granosa.