کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430043 1106537 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bovine WC1−γδT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-γ in response to exogenous IL-12 and IL-18
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Bovine WC1−γδT cells incubated with IL-15 express the natural cytotoxicity receptor CD335 (NKp46) and produce IFN-γ in response to exogenous IL-12 and IL-18
چکیده انگلیسی

SummaryThe γδT cells of ruminants are believed to participate in innate immunity and have been described with regulatory, inflammatory and cytotoxic functions. Here we describe a subset of CD3+ TcR1+ WC1−γδT cells expressing CD335 (NKp46), classically associated with CD3− natural killer (NK) cells, as a consequence of incubation with IL-15. This population, undetectable at the time of collection, developed after 2 week of IL-15 culture from splenic leukocytes (SPL) reaching greater than 50% of the total γδT cells. However, they did not grow well from peripheral blood leukocytes (PBL). Splenic γδT cells positively selected by magnetic separation prior to incubation with IL-15 and analyzed by flow cytometry, consistently yielded CD3+ cells expressing CD335. These cells arose from the CD335−γδT cell population suggesting that the new population represents up-regulation of CD335 by γδT cells. CD335 mRNA expression from sorted IL-15-incubated SPL CD335+γδT cells or NK cells exceeded that of CD335−γδT cells. Incubation with IL-12 and IL-18 increased the number of CD335+γδT cells in both the PBL and SPL fractions as compared to controls or IL-12 or IL-18 alone. In addition, CD335+γδT cells demonstrated a robust ability to produce IFN-γ in response to exogenous IL-12 and IL-18. Taken as a whole, we describe a new phenotypically distinct bovine γδT cell sub-population capable of participating in type 1 immune responses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 32, Issue 8, 2008, Pages 1002–1010
نویسندگان
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