کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2430108 | 1106542 | 2008 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and characterization of two STAT isoforms from Sf9 cells
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
SummaryIn invertebrates, the JAK–STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 32, Issue 7, 2008, Pages 814–824
Journal: Developmental & Comparative Immunology - Volume 32, Issue 7, 2008, Pages 814–824
نویسندگان
Maw-Sheng Yeh, Chia-Hsiung Cheng, Chih-Ming Chou, Ya-Li Hsu, Cheng-Ying Chu, Gen-Der Chen, Shui-Tsung Chen, Guang-Chao Chen, Chang-Jen Huang,