کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2430256 | 1106555 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Non-specific antiviral response detected in RNA-treated cultured cells of the sandfly, Lutzomyia longipalpis Non-specific antiviral response detected in RNA-treated cultured cells of the sandfly, Lutzomyia longipalpis](/preview/png/2430256.png)
Lutzomyia longipalpis is the principal vector of visceral leishmaniasis in the Americas, and can also transmit some viruses. To help develop a gene-silencing system for this sandfly, we transfected cultured embryonic cells with various double-stranded RNAs using West Nile virus (WNV) virus-like particles (VLPs) expressing luciferase as the target RNA to demonstrate effective gene knock-down. When luciferase dsRNA was introduced into these cells, they produced the expected reduction in VLP-encoded luciferase, suggesting specific silencing of the luciferase gene. Surprisingly, we found that unrelated dsRNAs, which included those specific for several L. longipalpis gene sequences and Escherichia coli β-galactosidase, diminished replication of the VLP-encoded genome. These results are the first indication for a nucleic acid-induced, non-specific antiviral response in this important insect vector.
Journal: Developmental & Comparative Immunology - Volume 32, Issue 3, 2008, Pages 191–197