کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430609 1553620 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptome analysis of the unfolded protein response in hemocytes of Litopenaeus vannamei
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Transcriptome analysis of the unfolded protein response in hemocytes of Litopenaeus vannamei
چکیده انگلیسی


• DsRNA against Bip was injected to activating UPR in Litopenaeus vannamei.
• Transcriptome analysis was carried out, and 200,230 unigenes were obtained.
• L. vannamei UPR was involved in various metabolic processes.
• L. vannamei UPR was engaged in immune-associated signaling pathways.

In this study, Litopenaeus vannamei was injected with double-stranded RNA (dsRNA) against L. vannamei immunoglobulin heavy chain binding protein (LvBip) to activating UPR in the hemocytes, shirmps injected dsRNA against enhanced green fluorescence protein (eGFP) as control group. And genes expression in hemocytes of then were analyzed using Illumina Hiseq 2500 (PE100). By comparing the analyzed results, 1418 unigenes were significantly upregulated, and 596 unigenes were significantly down-regulated upon UPR. Analysis of the differentially expressed genes against known databases indicated that the distribution of gene pathways between the upregulated and down-regulated genes were substantially different. A total of 208 genes of UPR system were obtained, and 69 of them were differentially expressed between the two groups. Results also showed that L. vannamei UPR was involved in various metabolic processes, such as glycometabolism, lipid metabolism, amino acid metabolism, and nucleic acid metabolism. In addition, UPR was emgaged in immune-assicoated signaling pathways, such as NF-κB signaling pathway, NOD-like receptor signaling pathway, Hippo signaling pathway, p38 MAPK signaling pathway and Wnt signaling pathway in L. vannamei. These results improved our current understanding of the L. vannamei UPR, and highlighted its importance in cell homeostasis upon environmental stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 54, July 2016, Pages 153–163
نویسندگان
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