کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430779 1553619 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
First characterization of three cyclophilin family proteins in the oyster, Crassostrea ariakensis Gould
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
First characterization of three cyclophilin family proteins in the oyster, Crassostrea ariakensis Gould
چکیده انگلیسی


• We firstly identified three cyclophilins (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3 from the oyster, C. ariakensis.
• All the Ca-CyPs contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide.
• Tissue distribution studies revealed that Ca-CyPs were expressed in all examined tissues with the highest levels in hemocytes.
• RLO incubation increased the expression levels of Ca-CyPs, indicating Ca-CyPs might be involved in oyster immune response.

Cyclophilins (CyPs) are a family of proteins that bind the immunosuppressive agent cyclosporin A (CsA) with high-affinity and belong to one of the three superfamilies of peptidyl-prolyl cis-trans isomerases (PPIase). In this report, three cyclophilin genes (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3, were identified from oyster, Crassostrea ariakensis Gould in which Ca-CyPA encodes a protein with 165 amino acid sequences, Ca-CyPB encodes a protein with 217 amino acid sequences and Ca-PPIL3 encodes a protein with 162 amino acid sequences. All of the three Ca-CyPs genes contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide sequences. Tissue distribution study revealed that Ca-CyPs were ubiquitously expressed in all examined tissues and the highest levels were observed in hemocytes. RLO incubation upregulated the mRNA expression levels of Ca-CyPs, indicating that three Ca-CyPs might be involved in oyster immune response against RLO infection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 55, August 2016, Pages 257–266
نویسندگان
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