LECT2 improves the outcomes in ayu with Vibrio anguillarum infection via monocytes/macrophages

• We reported the beneficial effect of LECT2 on fish defense against pathogens.
• rPaLECT2 administration improved the outcomes of Vibrio anguillarum infected ayu.
• We depleted ayu monocytes/macrophages by clodronate-liposomes treatment.
• rPaLECT2 mediated antibacterial protection via monocytes/macrophages in ayu.

Leukocyte cell-derived chemotaxin 2 (LECT2) is reported to be a cytokine involved in the immune response against pathogenic microorganisms in fish. However, its accurate function in whole fish remains unclear. In this study, we provide the first report on the effect of LECT2 on fish defenses against pathogens in vivo. The administration of recombinant LECT2 improved the survival rate of Vibrio anguillarum infected ayu. The bacterial burden of V. anguillarum infected ayu was decreased in LECT2-treated ayu blood, liver, spleen, and kidney compared with saline control. In bacteria-infected ayu, LECT2 treatment altered the mRNA expression of cytokines, including TNFα, IL-1β, and IL-10, which are all important for the inflammatory response in fish. LECT2 treatment also reduced histological damage in bacteria-infected ayu, and increased peritoneal monocytes/macrophages in both healthy and infected ayu at 12 h post infection. When ayu monocytes/macrophages were depleted by clodronate-liposomes treatment, LECT2 treatment did not increase the survival rate of bacteria-infected fish compared with healthy control fish. Thus our results suggest that LECT2 can modulate host defense in ayu and mediate antibacterial protection against V. anguillarum through monocytes/macrophages.