کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2431163 1106745 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Oxidative stress response of the black tiger shrimp Penaeus monodon to Vibrio parahaemolyticus challenge
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Oxidative stress response of the black tiger shrimp Penaeus monodon to Vibrio parahaemolyticus challenge
چکیده انگلیسی


• Vibrio parahaemolyticus damaged the ultrastructure of hepatopancreas in Penaeus monodon.
• V. parahaemolyticus induced the respiratory burst and ROS production in P. monodon.
• Antioxidant enzyme activities were significantly affected by V. parahaemolyticus challenge.

Vibrio parahaemolyticus is a virulent pathogen that affects shrimp aquaculture. Reactive oxygen species are produced by the immune system that defends the host against foreign microorganisms. In the present study, the oxidative stress response in hepatopancreas and gills of Penaeus monodon to V. parahaemolyticus challenge were studied, such as respiratory burst, ROS production (·O2− and ·OH), activities of antioxidant enzymes (CAT, GPx, SOD, POD and GST) and oxidative damage to lipid and protein (indexed by contents of MDA). Compared with the control group, after V. parahaemolyticus challenge, respiratory burst and ROS production were up-regulated significantly. GPx and POD activity increased significantly in hepatopancreas and gills of the shrimps at 12 h, but CAT activity decreased markedly at 12 h and 24 h. SOD and GST activity in hepatopancreas of the shrimps increased significantly at 1.5 h, but decreased markedly at 12 h–48 h. MDA content increased significantly after 6 h–24 h challenge. HE staining showed that V. parahaemolyticus challenge induced damage symptoms in hepatopancreas of P. monodon. Our study revealed that V. parahaemolyticus influenced the antioxidative status and caused oxidative stress and tissue damage via confusion of antioxidant enzymes in P. monodon.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 46, Issue 2, October 2015, Pages 354–365
نویسندگان
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