Cloning and functional characterisation of a peroxiredoxin 1 (NKEF A) cDNA from Atlantic salmon (Salmo salar) and its expression in fish infected with Neoparamoeba perurans

Peroxiredoxin 1 (Prx 1), also known as natural killer enhancing factor A (NKEF A), has been implicated in the immune response of both mammals and fish. Amoebic gill disease (AGD), caused by Neoparamoeba perurans, is a significant problem for the Atlantic salmon (Salmo salar L.) aquaculture industry based in Tasmania, Australia. Here we have cloned and functionally characterized a Prx 1 open reading frame (ORF) from Atlantic salmon liver and shown that Prx 1 gene expression was down-regulated in the gills of Atlantic salmon displaying symptoms of AGD. The Prx 1 ORF encoded all of the residues and motifs characteristic of typical 2-Cys Prx proteins from eukaryotes and the recombinant protein expressed in Escherichia coli catalyzed thioredoxin (Trx)-dependent reduction of H2O2, cumene hydroperoxide (CuOOH) and t-butyl hydroperoxide (t-bOOH) with Km values of 122, 77 and 91 μM, respectively, confirming that it was a genuine 2-Cys Prx. The recombinant protein also displayed a double displacement reaction mechanism and a catalytic efficiency (kcat/Km) with H2O2 of 1.5 × 105 M−1 s−1 which was consistent with previous reports for the 2-Cys Prx family of proteins. This is the first time that a Prx 1 protein has been functionally characterized from any fish species and it paves the way for further investigation of this important 2-Cys Prx family member in fish.

► Peroxiredoxin 1 (Prx 1) has been implicated in the immune response of fish.
► Amoebic gill disease (AGD) is a problem for Atlantic salmon culture in Australia.
► Prx 1 gene expression is down-regulated in AGD-affected Atlantic salmon gill.
► Recombinant Atlantic salmon Prx 1 displays a double displacement reaction mechanism.
► Recombinant Atlantic salmon Prx 1 has a kcat/Km with H2O2 of 1.5 × 105 M−1 s−1.