کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2431857 1106774 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization and comparative analyses of two amphioxus intelectins involved in the innate immune response
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Characterization and comparative analyses of two amphioxus intelectins involved in the innate immune response
چکیده انگلیسی

Intelectin is a new type of soluble galactofuranose-binding lectin involved in innate immunity. Here we report another intelectin homolog, AmphiITLN239631, obtained from amphioxus, the transitional form between vertebrates and invertebrates. AmphiITLN239631 encoded 396 amino acids with a highly conserved fibrinogen-related domain (FReD), An intelectin domain and a putative Collagen domain. AmphiITLN239631 was ubiquitously expressed in all tissues we tested and transcripts in skin increased after challenge of both Escherichia coli and Staphylococcus aureus, although in different levels. Recombinant AmphiITLN239631 expressed in E. coli system could agglutinate both Gram-positive and Gram-negative bacteria in a calcium independent manner. Furthermore, recombinant protein was able to bind to lipopolysaccharide (LPS) and peptidoglycan (PGN), the major components of Gram-positive and Gram-negative bacteria cell walls, respectively.We also compared AmphiITLN239631 with previously identified AmphiITLN71469 and found that their tissue specificities, expression patterns upon bacteria challenge, and polysaccharide-binding affinities etc vary considerably. Our results could provide insight into the evolution and function of the intelectin family.


► Another intelectin homolog AmphiITLN239631 was identified in amphioxus.
► AmphiITLN239631 were expressed in major tissues/organs at different levels.
► AmphiITLN239631 transcripts in skin increased after bacteria challenges.
► Recombinant AmphiITLN239631 bound to LPS/PGN and induced bacterial agglutination.
► Properties of AmphiITLN239631 and AmphiITLN71469 varied considerably.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 34, Issue 5, May 2013, Pages 1139–1146
نویسندگان
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