Alpha2-macroglobulin from an Atlantic shrimp: Biochemical characterization, sub-cellular localization and gene expression upon fungal challenge

In this study, we report on the isolation and characterization of an alpha2-macroglobulin (α2M) from the plasma of the pink shrimp Farfantepenaeus paulensis, its sub-cellular localization and transcriptional changes after infection by fungi. The molecular mass of the α2M was estimated at 389 kDa by gel filtration and 197 kDa by SDS-PAGE, under reducing conditions, suggesting that α2M from F. paulensis consists of two identical sub-units, covalently linked by disulphide bonds. The N-terminal amino acid sequence of the α2M from F. paulensis was very similar to those of other penaeid shrimps, crayfish and lobster (70–90% identity) and to a less extent with that of freshwater prawn (40% identity). A monoclonal antibody raised against the Marsupenaeus japonicus α2M made it possible to demonstrate that α2M of F. paulensis is stored in the vesicles of the shrimp granular hemocytes (through immunogold assay). Quantitative real-time PCR (qPCR) analysis showed that α2M mRNA transcripts significantly increased 24 h after an experimental infection with the shrimp pathogen Fusarium solani and it returned to the basal levels at 48 h post-injection. This is the first report on a α2M characterization in an Atlantic penaeid species and its expression profile upon a fungal infection.

► We report the pink shrimp α2M characterization and its expression profile upon a fungal infection.
► α2M consists of two identical sub-units of 197 kDa, covalently linked by disulphide bonds.
► α2M N-terminal has high identity (70–90%) with other α2Ms from shrimps, crayfish and lobster.
► Crustacean α2M is stored in the vesicles of the granular hemocytes.
► α2M mRNA increases 24 h after Fusarium solani challenge and it returned to basal levels at 48 h.