کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2431967 1106775 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Over-expression, purification and immune responses to Aeromonas hydrophila AL09-73 flagellar proteins
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Over-expression, purification and immune responses to Aeromonas hydrophila AL09-73 flagellar proteins
چکیده انگلیسی

Aeromonas hydrophila is ubiquitous in aquatic environments worldwide and causes many diseases in fish as well as human. Recent outbreaks of aeromonad diseases in channel catfish prompted us to investigate catfish immune responses during infection of A. hydrophila. In this communication, we report to amplify, over-express, purify and characterize 19 A. hydrophila flagellar proteins. All recombinant proteins were confirmed by nucleotide sequencing of expression plasmids, SDS-PAGE analysis and His tag Western blot of induced proteins. Our preliminary result also showed that the purified recombinant FlgK protein reacted strongly to sera from experimentally infected catfish, suggesting that this protein has potential for a novel target for vaccine development. It is also anticipated that these recombinant proteins will provide us with very useful tools to investigate host immune response to this microorganism.


► Aeromonas hydrophila flagellar proteins were expressed in E. coli expression system.
► Recombinant A. hydrophila flagellar proteins were purified by nickel affinity chromatography.
► Experimentally infected catfish sera reacted strongly to the FlgK protein.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 31, Issue 6, December 2011, Pages 1278–1283
نویسندگان
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