کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2432083 | 1106779 | 2013 | 8 صفحه PDF | دانلود رایگان |
• Effective ex vivo assay for pathogen-probiotic-host interaction in warm-water fish.
• More pathogen and probiotic colonized anterior intestine than posterior parts.
• Pathogen and probiotic competed for the adhesive sites in tilapia intestines.
• Induced expression of immune cytokines in the anterior sac by pathogen or probiotic.
• Lactobacillus plantarum JCM 1149 took the protective effects against Aeromonas hydrophila NJ-1 attack.
To investigate the ex vivo interactions of probiotic-pathogen-host in warm-water fish, hybrid tilapia (Oreochromis niloticus♀ × Oreochromis aureus♂) were sacrificed to isolate anterior and posterior intestine for incubation with phosphate-buffered saline (PBS; pH 7.2) as the control, Lactobacillus plantarum JCM 1149 at 1.0 × 109 CFU/ml, Aeromonas hydrophila NJ-1 at 1.0 × 108 CFU/ml, or the both combination. Denaturing Gradient Gel Electrophoresis (DGGE) fingerprint and consequent sequence analysis confirmed anterior intestine sac was more prone to the colonization of L. plantarum JCM 1149 and A. hydrophila NJ-1 than the posterior part. L. plantarum JCM 1149 and A. hydrophila NJ-1 inhibited the population each other in anterior or posterior sac, indicating their competition for the colonization. The induced expression of HSP70, IL-1β and TNF-α in the anterior sac by the addition of L. plantarum JCM 1149 or A. hydrophila NJ-1 demonstrated the activity and a local immune response of ex vivo anterior sac. Compared with posterior intestine, higher population colonization and more sensitive immune response of anterior sac indicated differential patterns for the probiotic-pathogen-host interactions. Scanning electronic microscopy (SEM) observation showed that pathogen A. hydrophila NJ-1 damaged the anterior intestine, which was alleviated by the pretreatment of L. plantarum JCM 1149, showing its probiotic effect.
Journal: Fish & Shellfish Immunology - Volume 35, Issue 1, July 2013, Pages 146–153