کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2432090 | 1106779 | 2013 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus) Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus)](/preview/png/2432090.png)
• Cathepsin H cDNA was identified from a rock bream.
• RbCTSH had a well-conserved EPQNCSAT region within the aligned sequence.
• RbCTSH gene was predominantly in the intestine, spleen, head kidney and stomach.
• RbCTSH was up-regulated in the fish infected with several pathogens.
Cathepsins are lysosomal cysteine proteases belonging to the papain family, whose members play important roles in normal metabolism for the maintenance of cellular homeostasis. Rock bream (Oplegnathus fasciatus) cathepsin H (RbCTSH) cDNAs were identified by expressed sequence tag analysis of a lipopolysaccharide-stimulated rock bream liver cDNA library. The full-length RbCTSH cDNA (1326 bp) contained an open reading frame of 978 bp encoding 325 amino acids. The presence of an ERFNIN-like motif was predicted in the propeptide region of RbCTSH. Furthermore, multiple alignments showed that the EPQNCSAT region was well conserved among other cathepsin H sequences. Phylogenetic analysis revealed that RbCTSH is most closely related to Nile tilapia cathepsin H. RbCTSH was expressed significantly in the intestine, spleen, head kidney and stomach. RbCTSH mRNA expression was also examined in several tissues under conditions of bacterial and viral challenge. All examined tissues of fish infected with Edwardsiella tarda, Streptococcus iniae and red sea bream iridovirus (RSIV) showed significant increases in RbCTSH expression compared to the control. In the kidney and spleen, RbCTSH mRNA expression was upregulated markedly following infection with bacterial pathogens. These findings indicate that RbCTSH plays an important role in the innate immune response of rock bream. Furthermore, these results provide important information for the identification of other cathepsin H genes in various fish species.
Journal: Fish & Shellfish Immunology - Volume 35, Issue 1, July 2013, Pages 188–194