کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2432124 | 1106782 | 2011 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Gene expression profiling in respond to TBT exposure in small abalone Haliotis diversicolor Gene expression profiling in respond to TBT exposure in small abalone Haliotis diversicolor](/preview/png/2432124.png)
In this study, we investigated the gene expression profiling of small abalone, Haliotis diversicolor by tributyltin (TBT) exposure using a cDNA microarray containing 2473 unique transcripts. Totally, 107 up-regulated genes and 41 down-regulated genes were found. For further investigation of candidate genes from microarray data and EST analysis, quantitative real-time PCR was performed at 6 h, 24 h, 48 h, 96 h and 192 h TBT exposure. 26 genes were found to be significantly differentially expressed in different time course, 3 of them were unknown. Some gene homologues like cellulose, endo-beta-1,4-glucanase, ferritin subunit 1 and thiolester containing protein II CG7052-PB might be the good biomarker candidate for TBT monitor. The identification of stress response genes and their expression profiles will permit detailed investigation of the defense responses of small abalone genes.
► We investigated the gene expression profiling of small abalone by TBT exposure.
► 107 up-regulated genes and 41 down-regulated genes were found by microarray.
► 26 significantly differentially expressed genes were found by real-time PCR.
► Some gene homologues might be the good biomarker candidate for TBT monitor.
Journal: Fish & Shellfish Immunology - Volume 31, Issue 4, October 2011, Pages 557–563