کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2432137 | 1106783 | 2012 | 10 صفحه PDF | دانلود رایگان |
Intelectin is a recently described galactofuranose-binding lectin that plays a role in innate immunity in vertebrates. Little is known about intelectin in invertebrates, including amphioxus, the transitional form between vertebrates and invertebrates.We cloned an amphioxus intelectin homolog, AmphiITLN-like, coding 302 amino acids with a conserved fibrinogen-related domain (FReD) in the N-terminus and an Intelectin domain in the C-terminus. In situ hybridization in adult amphioxus showed that AmphiITLN-like transcripts were highly expressed in the digestive tract and the skin. Quantitative real-time PCR revealed that AmphiITLN-like is significantly up-regulated in response to Staphylococcus aureus challenge, but only modestly to Escherichia coli. In addition, recombinant AmphiITLN-like expressed in E. coli agglutinates Gram-negative and Gram-positive bacteria to different degrees in a calcium dependent manner. Recombinant AmphiITLN-like could bind lipopolysaccharide (LPS) and peptidoglycan (PGN), the major cell wall components of Gram-negative and Gram-positive bacteria, respectively, with a higher affinity to PGN.Our work identified and characterized for the first time an amphioxus intelectin homolog, and provided insight into the evolution and function of the intelectin family.
► One homolog of intelectin named AmphiITLN-like was identified in amphioxus.
► AmphiITLN-like transcripts were mainly expressed in digestive tract.
► The up-regulation of AmphiITLN-like occurred within 2–8 h after bacteria challenge.
► Recombinant AmphiITLN-like recognized PGN, LPS and induced bacterial agglutination.
► AmphiITLN-like recognizing PGN/Staphylococcus aureus more intensely than LPS/Escherichia coli was found.
Journal: Fish & Shellfish Immunology - Volume 33, Issue 1, July 2012, Pages 11–20