کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2432214 | 1106786 | 2011 | 8 صفحه PDF | دانلود رایگان |

Atrazine (ATR) and chlorpyrifos (CPF) are widely used in agriculture has resulted in a series of toxicological and environmental problems. The aim of this study was to investigate the effects of ATR, CPF and their mixture on the mRNA levels of interleukin-1β (IL-1β), interleukin receptor I (IL-1RI) and interferon gamma (IFN-γ2b) in both spleen and head kidney of Common carp. In this study, juvenile common carp were exposed to ATR (at concentrations of 4.28, 42.8 and 428 μg/L), CPF (at concentrations of 1.16, 11.6 and 116 μg/L), and their mixture (at concentrations of 1.16, 11.6 and 116 μg/L). The mRNA levels of IL-1β, IL-1R1 and IFN-γ2b in spleen and head kidney were detected by using RT-PCR. Our results indicated that IL-1β, IL-1R1 expression significantly increased after exposure in high concentration ATR, CPF and their mixture, but IFN-γ2b mRNA shown different expression trends. Our results suggested that ATR, CPF and their mixture probably induced damages on spleen and head kidney may be association with increasing IL-1β, IL-1R1 mRNA synthesis. After 20-day recovery test, IL-1β, IL-1R1 and IFN-γ2b mRNA expression remain at high level in majority of the treated groups, we concluded that the restoration of tissue and immune system damage probably needs longer time.
► We model pesticides poisoning on immune organs of common carp.
► We examine changes in the level of IL-1β, IL-1R1 and IFN-γ2b mRNA.
► IL-1β, IL-1R1 expression increased after exposure in pesticides for 40 days.
► In recovery test, IL-1β, IL-1R1 and IFN-γ2b mRNA expression remain at high level.
► We conclude pesticides probably produced damages on immune organs of common carp.
Journal: Fish & Shellfish Immunology - Volume 31, Issue 1, July 2011, Pages 126–133