کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432262 1106788 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix
چکیده انگلیسی

Catalase (CAT) can effectively eliminate H2O2 and maintain the redox balance of immune system, which is essential for innate immunity. A catalase gene was cloned and its potential role in immune system was investigated in the clam, Meretrix meretrix. The catalase (MmeCAT) gene had an open reading frame of 1533 bp encoding 511 amino acids which showed high identity with that of molluscs. The distribution of MmeCAT in clam tissues was examined and the mRNA, protein expression and CAT activity paralleled with each other, with the highest expression in hepatopancreas. In response to H2O2 challenge, MmeCAT mRNA showed significantly higher expression at 12 h and 24 h post-challenge in experimental clams than in control clams (P < 0.05). Meanwhile, the protein expression in experimental clams was increased to about 3 times as much as that in control clams at 6 h post-challenge. After injection with a Vibrio parahaemolyticus-related bacterium (MM21), the expression of MmeCAT mRNA was significantly up-regulated at 12 h and 24 h post-injection (P < 0.05). It suggested that MmeCAT might be involved in the immune response to Vibrio infection. To better understand the role of MmeCAT in immune system, its mRNA expression was compared between a Vibrio-resistant population and a control population after immersion challenge with MM21. The continuously increased transcription in resistant population suggested MmeCAT could benefit the immune system of clams to defend against pathogen infection. Our study indicated that the redox balance was essential for M. meretrix to resist pathogen infection.


► A catalase gene was characterized from the clam Meretrix meretrix.
► The mRNA and protein expression were increased by H2O2 challenge.
►  Pathogen infection could significantly up-regulate the MmeCAT expression.
► Vibrio-resistant clam population had a higher MmeCAT expression than control population.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 34, Issue 1, January 2013, Pages 91–99
نویسندگان
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