کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432371 1106792 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Cloning and transcriptional analysis of two sialic acid-binding lectins (SABLs) from razor clam Solen grandis
چکیده انگلیسی

Sialic acid-binding lectin (SABL) plays crucial role in both innate and adaptive immune responses benefiting from its predominant affinity toward glycan. In the present study, two SABLs from razor clam Solen grandis (designated as SgSABL-1 and SgSABL-2) were identified, and their expression patterns, both in tissues and towards microorganism glycan stimulation, were then characterized. The cDNA of SgSABL-1 and SgSABL-2 was 988 and 1281 bp, containing an open reading frame (ORF) of 744 and 570 bp, respectively, and deduced amino acid sequences showed high similarity to other invertebrates SABLs. Both SgSABL-1 and SgSABL-2 encoded a C1q domain. SgSABL-1 and SgSABL-2 were found to be constitutively expressed in a wide range of tissues with different levels, including mantle, gill, gonad, hemocyte, muscle, and hepatopancreas, and both of them were highly expressed in hepatopancreas. SgSABL-1 and SgSABL-2 could be significantly induced after razor clams were stimulated by acetylated subunits-containing glycan LPS and PGN, suggesting the two SgSABLs might perform potential function of glycan recognition. In addition, SgSABL-2 could also be induced by β-1,3-glucan. All these results indicated that SgSABL-1 and SgSABL-2 might be involved in the immune response against microbe infection and contributed to the pathogens recognition.


► We identified two novel SABLs genes from Solen grandis.
► SgSABL-1 and SgSABL-2 exhibited different expression patterns in tissues.
► Both SABLs could be induced by microorganism glycan with different levels.
► SgSABL-1 and SgSABL-2 might be involved in pathogens recognition.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 32, Issue 4, April 2012, Pages 578–585
نویسندگان
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