کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2432386 | 1106793 | 2012 | 9 صفحه PDF | دانلود رایگان |

Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides previously identified in various crustaceans. Out of five isoforms identified in Penaeus monodon, ALFPm3 is the best characterized, exhibits antibacterial and antifungal activities and can protect the shrimp from viral infections. Herein, the most recent identified ALFPm, called ALFPm6, is characterized for its potential role in the shrimp’s immunity. RNA interference-mediated gene silencing was used to study the function of ALFPm6 in comparison to ALFPm3. Knockdown of ALFPm3 gene led to rapid death with a cumulative shrimp mortality of 86% within 7 days, accompanied by a 12- and 50-fold higher bacterial count after 2 days in the haemolymph and hepatopancreas, respectively, compared to the control shrimp injected with GFP dsRNA. In contrast, gene silencing of ALFPm6 alone had no effect on the shrimp mortality, but led to a significant increase in the cumulative mortality and a faster mortality rate following Vibrio harveyi and white spot syndrome virus (WSSV) infections, respectively. These results support the roles of ALFPm6 and ALFPm3 in the protection of shrimp against microbial infections.
► We reported the sequence and the genomic structure of ALFPm6 gene from Penaeus monodon.
► An importance of ALFPm3 in shrimp defense was shown by a rapid death of shrimp after ALFPm3 gene silencing.
► ALFPm6-silenced shrimp had a higher mortality rate upon challenge with Vibrio harveyi as compared to the control shrimp.
► Here, the crucial roles of ALFPm3 and ALFPm6 in shrimp defense against microbial infection were revealed.
Journal: Fish & Shellfish Immunology - Volume 32, Issue 1, January 2012, Pages 26–34