کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2432404 | 1106793 | 2012 | 10 صفحه PDF | دانلود رایگان |

The complement system is important in both innate and adaptive host defense against microbial infection in vertebrates. It contains three pathways: the classical, alternative, and lectin pathways. Complement component factors B and D are two crucial proteases in the alternative pathway. In this study, the genes of complement factors Bf/C2 and Df from channel catfish, Ictalurus punctatus were identified and characterized. Two complement factor B-related genes, Bf/C2A and Bf/C2B, and factor D gene Df were identified. Phylogenetic analysis suggested that Bf/C2A and Bf/C2B is likely orthologous to factor B and factor C2, respectively. Southern blot results suggested that these three genes are all single-copy genes in the catfish genome. The catfish Bf/C2A, Bf/C2B and Df genes were genetically mapped on linkage group 3, 20 and 29, respectively. Bf/C2A and Bf/C2B are highly expressed in liver and kidney, while Df is highly expressed in gill and spleen. After infection with Edwardsiella ictaluri, the expression of Bf/C2A, Bf/C2B and Df genes were found to be remarkably induced in the gill, liver, spleen and kidney at some sampling times, indicating that these three complement factors play a pivotal role in immune responses after the bacterial infection in catfish.
► Two complement factors Bf/C2 and Df were identified from channel catfish.
► The complement factors were characterized and genetically mapped.
► Bf/C2A, Bf/C2B and Df genes were all one copy gene in the channel catfish genome.
► Bf/C2A and Bf/C2B is likely orthologous to factor B and factor C2, respectively.
► Bf/C2A, Bf/C2B and Df genes were remarkably induced by bacteria infection.
Journal: Fish & Shellfish Immunology - Volume 32, Issue 1, January 2012, Pages 186–195