کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432408 1106793 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two classes of glutathione S-transferase genes with different response profiles to bacterial challenge in Venerupis philippinarum
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Two classes of glutathione S-transferase genes with different response profiles to bacterial challenge in Venerupis philippinarum
چکیده انگلیسی

Glutathione S-transferase (GST) is major cytosolic detoxification enzymes involved in many pathological and physiological processes. In the present study, two classes of GSTs (VpGST-1 and VpGST-2) were cloned from Venerupis philippinarum haemocytes by cDNA library and RACE approaches. Sequence alignments and phylogenetic analysis together supported that they belonged to a new member of sigma and pi classes GSTs protein family, respectively. The expression profiles of these two genes under Vibrio anguillarum challenge were investigated by quantitative RT-PCR. The bacterial challenge could significantly up-regulate the mRNA expression of both VpGST-1 and VpGST-2 with larger amplitude in VpGST-2, and the feedback speed for VpGST-2 was more rapid than that of VpGST-1. The differences in the response to bacterial challenge indicated that they were functional diversity and probably played cooperative roles in mediating the Vibrio challenge in clam.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 32, Issue 1, January 2012, Pages 219–222
نویسندگان
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