کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2432428 | 1106794 | 2011 | 6 صفحه PDF | دانلود رایگان |
Clonal ginbuna crucian carp is, a naturally gynogenetic fish, and is a useful model animal for studying T-cell-mediated immunity. To gain molecular information on MHC class I molecules from this species, we have identified four types of MHC class I (caauUA-S3n, caauUF-S3n, caauZE-S3n, and caauZB-S3n) and five beta 2-microglobulin (β2m) (caauβ2m-1a, caauβ2m-1b, caauβ2m-2, caauβ2m-3a and caauβ2m-3b) by an expressed sequence tag (EST) analysis and using homology cloning with degenerated primers. Like UA class I genes in other cyprinid fish, the caauUA-S3n shows features of classical MHC class I, such as conservation of all key amino acids interacting with antigenic peptides, and ubiquitous tissue expression. A phylogenetic analysis shows that the β2m-1 and β2m-2 isoforms are clustered with those of other cyprinid fishes, while β2m-3 isoforms make a cluster that is separated from a common ancestor of salmonid and cyprinid fishes. This finding suggests that the β2m isoforms of ginbuna cruician carp comprise two lineages and may possess different functions. The MHC class I and β2m sequences from one clonal strain will facilitate our understanding of the interaction of MHC class I with β2m in teleosts.
► We cloned four types of MHC class I and five β2m from a clonal ginbuna crucian carp.
► One of the MHC class I molecules shows features of classical MHC class I in several respects.
► The β2m isoforms comprise two lineages.
Journal: Fish & Shellfish Immunology - Volume 31, Issue 3, September 2011, Pages 469–474