کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2432564 | 1106798 | 2010 | 5 صفحه PDF | دانلود رایگان |
Differential leukocyte counts were taken of blood smears collected from laboratory stressed adult Australian swellsharks Cephaloscyllium laticeps. We calculated the granulocyte (combined count of heterophils and neutrophils) to lymphocyte (G/L) ratio to use as a new physiological indicator of stress for sharks. Animals were captured and stressed using commercial fishing gear (monofilament gillnet and mid-water longline) in a laboratory setting, with blood samples collected prior to capture and at predetermined intervals during a subsequent 72 h recovery period. There was a significant increase in the G/L ratio of 291.14 ± 54.13% at 72 h post-capture during recovery from the 6-h gillnet capture plus 15-mintue air exposure. Six hours of longline capture plus 15 min of air exposure also evoked a significant increase in the G/L ratio of 490.32 ± 294.25% (24 h post-capture) and 590.53 ± 277.65% (72 h post-capture). There was no significant change in the G/L ratio for control sharks that did not undergo capture stress but that experienced an identical blood-sampling regime as captured animals. Our study presents findings of stress-induced changes in leukocyte distribution within the peripheral blood of a shark species brought on by lymphopenia (decrease in lymphocytes) and granulocytosis (increase in granulocytes), and confirms this as a useful measure of the relatively rapid onset of stress in these animals.
Journal: Fish & Shellfish Immunology - Volume 29, Issue 3, September 2010, Pages 534–538