کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2432637 | 1106800 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification and expression localization of a Ran homologue in mollusc abalone, Haliotis diversicolor supertexta Identification and expression localization of a Ran homologue in mollusc abalone, Haliotis diversicolor supertexta](/preview/png/2432637.png)
Ran protein is a central molecular in several key nuclear functions, including nucleocytoplasmic transport, cell-cycle progression and nuclear envelope assembly. In this study, we have isolated and characterized a Ran homologue from a gastropod abalone which we named ab-Ran. The full-length cDNA consists of 1239 bp with an ORF encoding a 220 amino acid protein. The deduced amino acid sequence of ab-Ran shows highly similar to that of other Ran members (84–88%). Moreover, the ab-Ran contains five conserved regions and four carboxy-terminal residues CAAX-box. RT-PCR analysis showed that the ab-Ran was ubiquitously expressed in abalone tissues. The intracellular localization examined by immunofluorescence and immunohistochemistry staining displayed that ab-Ran was largely concentrated in the nuclei and partially in the cytoplasm. To the best of our knowledge, this is the first identification and characterization of a Ran homologue in mollusk.
Journal: Fish & Shellfish Immunology - Volume 30, Issue 3, March 2011, Pages 986–991