کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2433113 1106819 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila
چکیده انگلیسی

A strain of Aeromonas hydrophila associated with unusual mortalities in zebrafish (Danio rerio) culture facilities was isolated, identified and characterized. In challenge experiments, adult zebrafish were susceptible to infection by intraperitoneal (i.p.) injection with viable bacteria and its extracellular products (ECPs) reaching very high mortalities in a few hours. The infection, by the viable bacteria or the ECPs, caused cell death in kidney, due to the cytotoxic and haemolytic activities of the bacterial ECPs. Moreover, the infection affected the release of oxygen (ROS) and nitrogen (NO) reactive free radicals. To determine if this A. hydrophila infection induces an inflammatory response, mRNA expression levels of tumour necrosis factor-α (TNFα), interleukin-1β (IL-1β), interferon-γ (IFNγ) and inducible nitric oxide (iNOS) were assessed by real time PCR. The expression levels of TNFα, IL-1β and IFNγ were upregulated in the kidneys of infected zebrafish with viable bacteria, heat-killed bacteria and ECPs. Expression levels of iNOS were upregulated by ECPs. Mortality rate (LD50) and histopathology were also determined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 25, Issue 3, September 2008, Pages 239–249
نویسندگان
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