کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2433201 | 1106822 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: c-Lysozyme from Senegalese sole (Solea senegalensis): cDNA cloning and expression pattern c-Lysozyme from Senegalese sole (Solea senegalensis): cDNA cloning and expression pattern](/preview/png/2433201.png)
Lysozymes are key molecules of innate immunity and proved high bactericidal activity in fish, thus becoming attractive as tools for enhancing fish defences. In this study, a full-length c-type lysozyme cDNA from Senegalese sole (Solea senegalensis) has been cloned and characterized. The cDNA sequence was inferred from two overlapping fragments obtained by RACE-PCR and consisting on 631 bp coding for 143 aminoacids. Catalytic and other conserved residues required for lysozyme activity were identified. Pair wise alignments showed the higher identities with c-type lysozyme from other flatfish. Expression patterns under various conditions showed a basal level and a clear upregulation mostly in hematopoietic organs after stimulation with LPS or infection with Photobacterium damselae. This study represents a first step on the genetics and function of the c-lysozyme of Senegalese sole, though disclosing g-DNA structure, allelic variability and antibacterial activity must be requirements prior its immunological properties might have biotechnological applications.
Journal: Fish & Shellfish Immunology - Volume 25, Issue 5, November 2008, Pages 697–700