کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2433533 1106837 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced disease resistance in Artemia by application of commercial β-glucans sources and chitin in a gnotobiotic Artemia challenge test
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Enhanced disease resistance in Artemia by application of commercial β-glucans sources and chitin in a gnotobiotic Artemia challenge test
چکیده انگلیسی

The anti-infectious potential of a selection of putative immunostimulants including six commercial β-glucans (all extracted from baker's yeast Saccharomyces cerevisiae except for Laminarin) and chitin particles were verified in Artemia nauplii by challenging them under gnotobiotic conditions with the pathogen Vibrio campbellii. Under the described experimental conditions, no differential macroscopic nutritional effect (e.g. growth) was observed among the products. Significant increased survival was observed with β-glucan (Sigma) and Zymosan and to a lesser extent with MacroGard in challenged nauplii. A poor correlation was found between survival values of the challenged Artemia and the product compositions (such as chitin, mannose and β-glucan content) indicating that the quality of β-glucans (e.g. the ratio of β-1,3 and β-1,6 glucan, the molecular weight, the dimensional structure, type and frequency of branches), eventually in combination with other unidentified compounds, is more important than the amount of product offered. This small-scale testing under gnotobiotic conditions using freshly hatched Artemia nauplii allows for a rapid and simultaneous screening of anti-infectious and/or putative immunostimulatory polymers, and should be combined with studies on cellular and humoral immune responses in order to gain more quantitative insight into their functional properties.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 23, Issue 6, December 2007, Pages 1304–1314
نویسندگان
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