کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2435809 1107100 2007 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The molecular characterisation and antimicrobial activity of amidated bovine lactoferrin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
The molecular characterisation and antimicrobial activity of amidated bovine lactoferrin
چکیده انگلیسی

Chemical modification of bovine lactoferrin (LF) by amidation increased the net positive charge of the protein and markedly enhanced its activity against most Gram-positive and Gram-negative bacteria tested, but not against Saccharomyces cerevisiae or Penicillium candidum. Bovine lactoferrin was amidated with 1-ethyl-3-[3-(dimethylamino) propyl] carbodiimide in the presence of ammonium ions. Mass spectrometric analysis of the modified protein confirmed that amidation converted aspartyl or glutamyl residues to asparaginyl or glutaminyl residues. The changes in net charge and electrophoretic mobility of amidated LF were confirmed by electrophoretic and chromatographic methods. Isoionic point titration was used to determine changes to net charges and ion exchange chromatography results confirmed that the increased positive charge played an important role in the antimicrobial action of the amidated protein. Thus, chemical amidation of LF introduced substantial structural changes in the LF molecule, reflected in amino acid composition and increased net positive charge, and enhanced biological activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Dairy Journal - Volume 17, Issue 6, June 2007, Pages 606–616
نویسندگان
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