کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2435881 | 1107106 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of thermal treatment on the activation of bovine plasminogen
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effect of thermal treatment on the activation of bovine plasminogen Effect of thermal treatment on the activation of bovine plasminogen](/preview/png/2435881.png)
چکیده انگلیسی
The effect of heat on plasminogen (PG) was studied in both buffer and milk. The denaturation temperature for bovine PG was determined. Kinetic analysis of heated PG was conducted using a coupled assay and Spectrozyme®PL. The effect of heat on PG activation was studied in milk samples with and without addition of PG activator. The temperature range for denaturation of PG was between 50.1 and 61.6 °C. The overall catalytic efficiency increased for PG heated to 60 °C and above, as indicated by the significant increase in kcat/KM. In a milk system, a significant increase in activation of PG was measured at 57 °C. A drop in activation of PG was observed at higher temperatures, suggesting counteracting effects of other milk components that were affected by heat. Results indicated that the increased activation of heated bovine PG observed in buffer and milk systems was probably due to the unfolding of PG as a result of thermal denaturation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Dairy Journal - Volume 16, Issue 6, June 2006, Pages 580-585
Journal: International Dairy Journal - Volume 16, Issue 6, June 2006, Pages 580-585
نویسندگان
C.N. Burbrink, K.D. Hayes,