کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2435891 | 1107106 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effects of high pressure treatment on indigenous enzymes in bovine milk: Reaction kinetics, inactivation and potential application Effects of high pressure treatment on indigenous enzymes in bovine milk: Reaction kinetics, inactivation and potential application](/preview/png/2435891.png)
High pressure-induced inactivation of the indigenous milk enzymes alkaline phosphatase (ALP), γ-glutamyltransferase (GGT) and phosphohexoseisomerase (PHI) was studied in the pressure range 400–800 MPa at temperatures between 5 and 40 °C. With respect to pressure stability the following ranking was observed: ALP>GGT>PHI. PHI was inactivated after pressure treatment at 500 MPa and 20 °C for 10 min. In terms of reaction kinetics, inactivation of GGT followed first-order reaction kinetics in the range of 400–800 MPa whereas a reaction order of 1.5 was found for ALP. Reactivation of pressure-treated ALP was observed at low enzyme activity resulting from severe pressure treatment and 2 h storage at 35 °C. The influence of process temperature on the pressure-induced inactivation of GGT and ALP was limited in the range 5–40 °C.
Journal: International Dairy Journal - Volume 16, Issue 6, June 2006, Pages 655–661