کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2437167 1553760 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mismatch Repair mRNA and Protein Expression in Intestinal Adenocarcinoma in Sika Deer (Cervus nippon) Resembling Heritable Non-polyposis Colorectal Cancer in Man
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Mismatch Repair mRNA and Protein Expression in Intestinal Adenocarcinoma in Sika Deer (Cervus nippon) Resembling Heritable Non-polyposis Colorectal Cancer in Man
چکیده انگلیسی

SummaryIntestinal adenocarcinomas seen in an inbred herd of farmed sika deer (Cervus nippon) morphologically resembled human hereditary non-polyposis colorectal cancer (HNPCC). Features common to both included multiple de novo sites of tumourigenesis in the proximal colon, sessile and non-polyposis mucosal changes, the frequent finding of mucinous type adenocarcinoma, lymphocyte infiltration into the neoplastic tubules and Crohn's-like lymphoid follicles at the deep margin of the tumour. HNPCC is defined by a germline mutation of mismatch repair (MMR) genes resulting in their inactivation and loss of expression. To test the hypothesis that similar MMR gene inactivation occurs in the deer tumours, the expression of the four most important MMR genes, MSH2, MLH1, MSH6 and PMS2, was examined at the mRNA level by reverse transcriptase polymerase chain reaction (n = 12) and at the protein level by immunohistochemistry (n = 40) in tumour and control tissues. All four genes were expressed equally in normal and neoplastic tissues, so MMR gene inactivation could not be implicated in the carcinogenesis of this tumour in sika deer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Comparative Pathology - Volume 152, Issues 2–3, February–April 2015, Pages 131–137
نویسندگان
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