کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2438791 | 1107746 | 2006 | 7 صفحه PDF | دانلود رایگان |
SummaryThe expression of interleukin-1 (IL-1), tumour necrosis factor-α (TNF-α) and IL-6 were studied over a period of 35 days in the lungs of pigs experimentally infected with Mycoplasma hyopneumoniae, by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), morphometric analysis and in-situ hybridization. Fifteen colostrum-deprived pigs aged 14 days were inoculated intranasally with M. hyopneumoniae. IL-1, TNF-α and IL-6 were detected by RT-PCR in the lungs of the infected pigs from 7 days post-inoculation (dpi) onwards, but not in the uninfected control pigs. Concurrent expression of all three cytokines was always observed, in association with lung lesions. Inflammatory cytokine-positive cells were detected in the lungs at 7 dpi, their number increasing at 21 dpi, and decreasing thereafter. The results suggest that such cytokines play a role in mediating and regulating inflammation in M. hyopneumoniae infection.
Journal: Journal of Comparative Pathology - Volume 134, Issue 1, January 2006, Pages 40–46