کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2439272 1108094 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Short communication: Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Short communication: Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows
چکیده انگلیسی
Three adult lactating Holstein cows were injected in the subcutaneous abdominal vein with 175 ng/kg of body weight of Clostridium botulinum type C toxin (451 cow median toxic doses) to determine if this botulinum toxin crosses the blood-milk barrier. Whole blood (in sodium heparin) and clotted blood serum samples were taken at 0 min, 10 min, and 3, 6, 9, and 12 h postinoculation. Milk samples were taken at 0 min and at 3, 6, 9 and 12 h postinoculation. All samples were tested for the presence of the toxin using the mouse bioassay and immunostick ELISA test. The immunostick ELISA identified the toxin in whole blood and the mouse bioassay identified the toxin in serum at all times examined in all 3 animals. Toxin was not identified by either detection method in milk samples collected from the 3 animals. From these results, it appears that Clostridium botulinum type C toxin does not cross from the blood to the milk in detectable concentrations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 92, Issue 6, June 2009, Pages 2529-2533
نویسندگان
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