کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2441411 | 1108140 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of Polymerase Chain Reaction to Detect Adulteration of Sheep's Milk with Goats’ Milk
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of Polymerase Chain Reaction to Detect Adulteration of Sheep's Milk with Goats’ Milk Application of Polymerase Chain Reaction to Detect Adulteration of Sheep's Milk with Goats’ Milk](/preview/png/2441411.png)
چکیده انگلیسی
The polymerase chain reaction has been applied for the specific detection of goats’ milk in sheep's milk using primers targeting the mitochondrial 12S ribosomal RNA gene. The use of goat-specific primers yielded a 122-bp fragment from goats’ milk DNA, whereas no amplification signal was obtained in sheep's, cows’, and water buffaloes’ milk DNA. Polymerase chain reaction analysis of raw and heat-treated milk binary mixtures of sheep/goat enabled the specific detection of goats’ milk with a sensitivity threshold of 0.1%. This study demonstrates the usefulness of the proposed polymerase chain reaction assay for authentication of milk products in routine analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Dairy Science - Volume 88, Issue 9, September 2005, Pages 3115–3120
Journal: Journal of Dairy Science - Volume 88, Issue 9, September 2005, Pages 3115–3120
نویسندگان
I. López-Calleja, I. González, V. Fajardo, I. Martín, P.E. Hernández, T. García, R. Martín,