کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2454762 1110494 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptomic profiling as a screening tool to detect trenbolone treatment in beef cattle
ترجمه فارسی عنوان
پروفیل ترانسکتکتومی به عنوان یک ابزار غربالگری برای تشخیص درمان ترنبولون در گاو گوشتی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

The effects of steroid hormone implants containing trenbolone alone (Finaplix-H), combined with 17β-oestradiol (17β-E; Revalor-H), or with 17β-E and dexamethasone (Revalor-H plus dexamethasone per os) on the bovine muscle transcriptome were examined by DNA-microarray. Overall, large sets of genes were shown to be modulated by the different growth promoters (GPs) and the regulated pathways and biological processes were mostly shared among the treatment groups. Using the Prediction Analysis of Microarray program, GP-treated animals were accurately identified by a small number of predictive genes. A meta-analysis approach was also carried out for the Revalor group to potentially increase the robustness of class prediction analysis. After data pre-processing, a high level of accuracy (90%) was obtained in the classification of samples, using 105 predictive gene markers. Transcriptomics could thus help in the identification of indirect biomarkers for anabolic treatment in beef cattle to be applied for the screening of muscle samples collected after slaughtering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 96, Issue 3, June 2014, Pages 472–481
نویسندگان
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