کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2455029 1110503 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Formation of peste des petits ruminants spikeless virus-like particles by co-expression of M and N proteins in insect cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Formation of peste des petits ruminants spikeless virus-like particles by co-expression of M and N proteins in insect cells
چکیده انگلیسی

Peste des petits ruminants virus (PPRV) has a non-segmented negative sense RNA genome and is classified within the Morbillivirus genus of the Paramyxoviridae. Using the Bac-to-Bac® baculovirus expression system, we constructed recombinant baculoviruses that were able to co-express the PPRV matrix and nucleocapsid proteins in insect cells under the control of the polyhedron and p10 promoters, respectively. The results showed that although both structural proteins were expressed at a relatively low level, the interaction between them caused the formation of virus-like particles (VLPs) by viewing of transmission electron microscopy. The VLPs morphologically resembled authentic PPRVs but lacked spikes protruding from the particulate surfaces. Interestingly, the diameter of PPRV VLPs ranged from 100 to 150 nm, far less than the mean diameter (400–500 nm) of parental virions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 96, Issue 1, February 2014, Pages 213–216
نویسندگان
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