کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2456302 1110537 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and molecular characterization of a Brazilian strain of Borrelia anserina, the agent of fowl spirochaetosis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Isolation and molecular characterization of a Brazilian strain of Borrelia anserina, the agent of fowl spirochaetosis
چکیده انگلیسی

Borrelia anserina the agent of fowl spirochaetosis, has a worldwide distribution, where it is transmitted by Argas spp. ticks. The present study reports the first molecular characterization and in vitro isolation of an avian spirochaete strain from Brazil, presumably identified as B. anserina originated from naturally infected Argas miniatus ticks. DNA fragments of the rrs and flab genes were amplified by PCR and sequenced to determine phylogenetic similarities. The resulting sequences were 99.8% (483 of 484) and 98.7% (754 of 764) similar to GenBank corresponding sequences of B. anserina rrs and flaB genes, respectively. By neighbor-joining phylogenetic analysis, the flaB sequence of the Brazilian strain clustered in a monophyletic group with the sequence of B. anserina under 100% bootstrap support. The isolate was successfully isolated in BSK medium, with seven passages performed. The spirochaete strain isolated in the present study was genetically identified as B. anserina labeled as strain PL.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 83, Issue 2, October 2007, Pages 145–149
نویسندگان
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