Microsatellite markers for the analysis of genetic variability and relatedness in red-legged partridge (Alectoris rufa) farms in Spain

The usefulness of several microsatellites in the management of Alectoris rufa restocking farms is evaluated in the present report. We analysed seven microsatellite loci in 147 randomly chosen individuals from four Spanish farms. Global FIS values were not significant. Slight genetic differentiation was only found between Farms 1 and 4. The global first and second parent exclusionary powers were 0.742 and 0.930, respectively. Microsatellite analysis can estimate the genetic relatedness between pairs of individuals by means of the Identity index. Since genealogies are unknown in most farms, pairwise Identity index values proved to be helpful in the management of matings, and the improvement of reproductive success and fitness of the descendants. Mean Identity index values showed that individuals within farms were not genetically more related than expected under random mating. Variance of the Identity index values suggest the absence of closed familial groups in these farms.