کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2457911 1554413 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genotyping of the prion protein (PrP) gene in Red Maasai and Black Head Persian sheep in Tanzania
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Genotyping of the prion protein (PrP) gene in Red Maasai and Black Head Persian sheep in Tanzania
چکیده انگلیسی

Susceptibility to scrapie in sheep is largely influenced by four polymorphic amino acid positions of the ovine PrP gene at codons 136, 141, 154 and 171. Genotyping of corresponding DNA polymorphism can be used as a basis for selection decisions. A total of 100 Red Maasai and 79 Black Herd Persian sheep, representative of the widely distributed breeds in Tanzania, were genotyped by real-time PCR. Genomic DNA was extracted from buccal epithelial cells. We report six genotypes derived from four different alleles, with the ARQ/ARQ genotype being more frequent (p = 0.0081) in the Red Maasai than in the Black Head Persian sheep. Our study also demonstrated higher allelic frequency (p = 0.00055) of the ARQ in the Red Maasai than in Black Head Persian sheep, while the AHQ allelic frequency was higher (p = 0.00086) in the Black Head Persian. All the animals were homozygous LL141. The highly susceptible VRQ allele was not found in any of the sheep breeds examined in this study. Both breeds were genetically low-level resistant to scrapie (NSP3). Due to absence or very low frequency of the ARR allele in the two breeds, selection for genetic resistance to scrapie through an increase of the ARR allele does not seem very relevant. If new breeds of sheep are to be introduced for crossbreeding in Tanzania, care should be taken to avoid import of the VRQ allele. To our knowledge, this is the first study exploring the PrP genotypes in sub-Saharan African sheep and in the Red Maasai and Black Head Persian sheep breeds in particular. The data provide base-line information on genetic susceptibility of the two sheep breeds to scrapie, which may be useful in policy formulation for prevention and future research on prion diseases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 79, Issues 2–3, October 2008, Pages 146–151
نویسندگان
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